Journal: International Journal of Pharmaceutics: X

Article Title: Zein-based polysaccharide-tannic acid films as multifunctional barriers to prevent post-surgical adhesions

doi: 10.1016/j.ijpx.2026.100515

Figure Lengend Snippet: In vitro characterization: A) direct cells contact onto film surfaces and CLSM images after 6 days; indirect cell proliferation after 3 and 6 days towards: B) NHDF; C) Caco-2; D) CLSM images (in blue – nuclei; in green – cytoskeleton). (mean values ± SD; n = 3), ANOVA one-way; Scheffé test (* P value <0.05; ***P value <0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The in vitro characterization was carried out using normal human dermal fibroblasts (NHDF) from juvenile foreskin (PromoCell, VWR, Milan, Italy) and Human colorectal adenocarcinoma cells (Caco-2).

Techniques: In Vitro

Journal: Tissue Engineering Part A

Article Title: MicroRNA-1 and MicroRNA-206 Improve Differentiation Potential of Human Satellite Cells: A Novel Approach for Tissue Engineering of Skeletal Muscle

doi: 10.1089/ten.tea.2011.0191

Figure Lengend Snippet: FIG. 1. Satellite cell culture, differentiation, and characterization. During passages 8–15, cultured cells display a homoge- neous morphology, note the triangular-shaped cells that are typical for satellite cells, by differential interference contrast (DIC) microscopy (A). An immunofluorescent image of proliferating cells at passage 9 double stained for a satellite cell marker desmin (green) and a fibroblast marker MCA1399G (red). Nuclei are counterstained with DAPI (blue). More than 95% of the cells expressed the satellite cell marker desmin (B). Differentiation of confluent satellite cell cultures was induced by switching to differentiation medium. Five days after switching to differentiation medium, myotubes were clearly visible by DIC microscopy (C). All scale bars are 100 mm. Color images available online at www.liebertonline.com/tea

Article Snippet: The primary antibody consisted of either (1) a myogenic marker, rabbit-anti-human desmin (1:100; Novus Biological), (2) a fibroblast marker, mouse anti-human MCA1399G (1:100; AbD Serotec), (3) a sarcomere component, mouse-anti-human a-sarcomeric actin IgM (1:200; clone Alpha Sr-1; Abcam) (4) a myogenic transcription factor, mouse-anti-human MyoD (1:100; Dako), (5) a sarcomere component, mouse-anti-human myosin (MF20; 1:500), and (6) a satellite cell marker, mouse-anti-human Pax7 (1:10; both Developmental Studies Hybridoma Bank).

Techniques: Cell Culture, Microscopy, Staining, Marker

Journal: ACS Nano

Article Title: Multifunctional Injectable Bioadhesive with Toll-like Receptor 4 and Myeloid Differentiation Factor 2 Antagonistic Anti-inflammatory Potential for Periodontal Regeneration

doi: 10.1021/acsnano.4c15922

Figure Lengend Snippet: Biocompatibility of the iCPC@MgO composite hydrogel and its effects on hPDLSCs osteogenic differentiation. (A) CCK8 assay and (B) live/dead staining for hPDLSCs’ viability after culturing on the 4 mg mL –1 hydrogel in each well. (C) Alkaline phosphatase activity assay was used to evaluate the early osteogenic abilities after culturing on 4 mg mL –1 hydrogel at 4 and 7 days. (D) ARS at 21 days of culturing on the 4 mg mL –1 hydrogel. (E) Semiquantification of calcium nodules ( n = 6 biologically independent replicates). (F,G) Western blot assay to detect the protein levels of the Wnt/β-catenin pathway at 21 days of culturing on the hydrogel. (H) Runx2, β catenin, and GSK 3β relative mRNA expression in hPDLSCs at 21 days of culturing on the hydrogel. Data are mean ± s.d. Statistical significance was analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, ns represents nonsignificance).

Article Snippet: Human periodontal ligament cells (hPDLSCs) and human gingival fibroblasts (HGFs) were commercially obtained from the Elabscience Biotechnology Co. Ltd., Wuhan, China.

Techniques: CCK-8 Assay, Staining, ALP Activity Assay, Western Blot, Expressing